Coding

Part:BBa_K786032:Design

Designed by: Kaiyin Feng   Group: iGEM12_Hong_Kong-CUHK   (2012-10-24)


cas3 protein in E.coli's CRISPR system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1964
    Illegal PstI site found at 2302
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1581
    Illegal PstI site found at 1964
    Illegal PstI site found at 2302
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1964
    Illegal PstI site found at 2302
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1964
    Illegal PstI site found at 2302
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 214
    Illegal SapI site found at 904


Design Notes

We amplified the original segment of cas3 protein's coding dna sequence from E.coli K12 genome.


Source

E.coli K12 genome

References